Journal: Molecular cell

Article Title: Stresses that raise Np 4 A levels induce protective nucleoside tetraphosphate capping of bacterial RNA

doi: 10.1016/j.molcel.2019.05.031

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains BW25113 Datsenko and Wanner, 2000 N/A BW25113 Δ apaH This study N/A BW25113 Δ rppH Luciano et al., 2017 N/A BW25113 Δ apaH Δ rppH This study N/A BW25113 Δ apaH Δ araE Δ araH This study N/A BW25113 Δ apaH Δ rppH Δ araE Δ araH This study N/A BW25113 Δ yeiP Luciano et al., 2017 N/A BW25113 Δ trxB Richards et al., 2012 N/A BL21 (DE3) rne-131 Δ rna This study N/A MIC2067 Itaya et al., 1999 N/A Chemicals, Peptides, and Recombinant Proteins ApaH This study N/A ApaH-D37A This study N/A RppH Foley et al., 2015 N/A RppH-E53A Foley et al., 2015 N/A NudC This study N/A NudC-E178A This study N/A Tobacco acid pyrophosphatase Epicentre {"type":"entrez-nucleotide","attrs":{"text":"T19100","term_id":"601143","term_text":"T19100"}} T19100 Pce1 Luciano et al., 2017 N/A E. coli RNA polymerase Nudler et al., 2003 N/A LysU This study N/A T7 RNA polymerase Thermo Fisher EP0111 T4 DNA ligase Luciano et al., 2017 N/A Calf intestinal alkaline phosphatase New England Biolabs M0290L T4 polynucleotide kinase New England Biolabs M0201L rRNasin Promega N251B Inorganic pyrophosphatase Sigma-Aldrich I1643 T4 RNA ligase 1 New England Biolabs M0204 SuperScript III reverse transcriptase Thermo Fisher 18080-044 Nucleotides used in these studies Table S7 N/A Orthophosphoric acid, [ 32 P] Perkin Elmer NEX053S015MC TALON Metal Affinity Resin Clontech 635503 UNO Q1 column Bio-Rad 7200001 TLC PEI cellulose F Millipore 105579 3-acrylamidophenylboronic acid Ivanov et al., 2004 N/A Immobilon-Ny+ Millipore INYC00010 Sephadex G-25 GE Healthcare 17-0031-02 Phenol Fisher Scientific A92-500 Cadmium chloride Diamide Sigma-Aldrich D3648 L-arabinose Sigma-Aldrich A3256 Isopropyl β-D-1-thiogalactopyranoside (IPTG) GoldBio I2481 L-lysine Sigma-Aldrich L5626 Sucrose Sigma-Aldrich S0389 Rifampicin Sigma-Aldrich R3501 Oligonucleotides Oligonucleotides used in these studies Table S7 N/A Recombinant DNA Plasmids used in these studies Table S7 N/A Software and Algorithms ImageQuant TL GE Healthcare 29000737 ciCalc2 This study https://www.dropbox.com/sh/yni7ayej6iqz2mu/AADNwGYK__lnvwHHUDc_01d0a?dl=0 Open in a separate window KEY RESOURCES TABLE Disulfide stress induces widespread Np 4 capping of RNA transcripts in E. coli In vitro , Np 4 caps can be added by RNA polymerase or aminoacyl-tRNA synthetases Np 4 caps are removed in E. coli by ApaH and RppH, triggering rapid RNA degradation ApaH acts as both a sensor and an effector of disulfide stress, which inactivates it

Techniques: Recombinant, Software